Sci Rep:华南农大吴珍芳课题组发表转基因动物生物反应器研究成果

2017年1月24日，国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了华南农业大学动物科学学院/国家生猪种业工程技术研究中心吴珍芳教授题为“Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors”的研究论文。博士后曾芳为论文第一作者，吴珍芳教授为通讯作者。

论文在国际上首次报道了以唾液腺作为生物反应器，从转基因动物的唾液中生产出具有良好生物活性、可用于治疗人类小儿脑瘫和老年痴呆等神经损伤或退化性疾病的人神经生长因子蛋白。这标志着基于转基因动物唾液腺生物反应器制备人类蛋白药物技术的首次成功建立。该技术在生物医药领域具有重要的应用前景。

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原文摘要：

The salivary glands of animals have great potential to act as powerful bioreactors to produce human therapeutic proteins. Human nerve growth factor (hNGF) is an important pharmaceutical protein that is clinically effective in the treatment of many human neuronal and non-neuronal diseases. In this study, we generated 18 transgenic (TG) founder mice each carrying a salivary gland specific promoter-driven hNGF transgene. A TG mouse line secreting high levels of hNGF protein in its saliva (1.36 μg/mL) was seleced. hNGF protein was successfully purified from the saliva of these TG mice and its identity was verified. The purified hNGF was highly functional as it displayed the ability to induce neuronal differentiation of PC12 cells. Furthermore, it strongly promoted proliferation of TF1 cells, above the levels observed with mouse NGF. Additionally, saliva collected from TG mice and containing unpurified hNGF was able to significantly enhance the growth of TF1 cells. This study not only provides a new and efficient approach for the synthesis of therapeutic hNGF but also supports the concept that salivary gland from TG animals is an efficient system for production of valuable foreign proteins.